MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 108(3) May 2013
PAGES: 390-391 DOI: 10.1590/0074-0276108032013020 Short communication
A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia

Stephanie Wagner Gallo1, Patrícia Locosque Ramos2, Carlos Alexandre Sanchez Ferreira1, Sílvia Dias de Oliveira1,+

1Laboratório de Imunologia e Microbiologia, Departamento de Biologia Celular e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brasil
2Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, SP, Brasil

Abstract

Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.

Received 14 May 2012
Accepted 18 October 2012
Financial support: CAPES
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

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