PAGES: 390-391 DOI: 10.1590/0074-0276108032013020 Short communication
A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia

Stephanie Wagner Gallo1, Patrícia Locosque Ramos2, Carlos Alexandre Sanchez Ferreira1, Sílvia Dias de Oliveira1,+

1Laboratório de Imunologia e Microbiologia, Departamento de Biologia Celular e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brasil
2Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, SP, Brasil


Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.

Received 14 May 2012
Accepted 18 October 2012
Financial support: CAPES
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.


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