MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 108(3) May 2013
PAGES: 359-367 DOI: 10.1590/0074-0276108032013015 Full paper
Sequence polymorphisms in Pvs48/45 and Pvs47 gametocyte and gamete surface proteins in Plasmodium vivax isolated in Korea

Mi Kyung Woo1, Kyeong Ah Kim1, JuYeon Kim1, Jun Seo Oh2, Eun Taek Han3, Seong Soo A An4, Chae Seung Lim1,+

1Department of Laboratory Medicine, Korea University Guro Hospital
2Laboratory of Cellular Oncology, Brain Korea 21 Graduate School of Medicine, College of Medicine, Korea University, Seoul, Republic of Korea
3Department of Parasitology, Kangwon National University College of Medicine, Chuncheon, Republic of Korea
4Department of BioNano Technology, Gachon BioNano Research Institute,
Gachon University, Seongnam-si, Gyeonggi Do, Republic of Korea

Abstract

Nucleotide sequence analyses of the Pvs48/45 and Pvs47 genes were conducted in 46 malaria patients from the Republic of Korea (ROK) (n = 40) and returning travellers from India (n = 3) and Indonesia (n = 3). The domain structures, which were based on cysteine residue position and secondary protein structure, were similar between Plasmodium vivax (Pvs48/45 and Pvs47) and Plasmodium falciparum (Pfs48/45 and Pfs47). In comparison to the Sal-1 reference strain (Pvs48/45, PVX_083235 and Pvs47, PVX_083240), Korean isolates revealed seven polymorphisms (E35K, H211N, K250N, D335Y, A376T, I380T and K418R) in Pvs48/45. These isolates could be divided into five haplotypes with the two major types having frequencies of 47.5% and 20%, respectivelfy. In Pvs47, 10 polymorphisms (F22L, F24L, K27E, D31N, V230I, M233I, E240D, I262T, I273M and A373V) were found and they could be divided into four haplotypes with one major type having a frequency of 75%. The Pvs48/45 isolates from India showed a unique amino acid substitution site (K26R). Compared to the Sal-1 and ROK isolates, the Pvs47 isolates from travellers returning from India and Indonesia had amino acid substitutions (S57T and I262K). The current data may contribute to the development of the malaria transmission-blocking vaccine in future clinical trials.

Received 9 August 2012
Accepted 9 October 2012
Financial support: Korea University, R & D Grant (2011)
MKW and KAK contributed equally to this work.
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

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