PAGES: 321-327 DOI: 10.1590/0074-0276108032013010 Full paper
Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil

Marcio Roberto Silva1,2,+ , Adalgiza da Silva Rocha3, Ronaldo Rodrigues da Costa4, Andrea Padilha de Alencar5, Vania Maria de Oliveira1, Antônio Augusto Fonseca Júnior5, Mariana Lázaro Sales5, Marina de Azevedo Issa5, Paulo Martins Soares Filho5, Omara Tereza Vianello Pereira5, Eduardo Calazans dos Santos5, Rejane Silva Mendes5, Ângela Maria de Jesus Ferreira5, Pedro Moacyr Pinto Coelho Mota5, Philip Noel Suffys3, Mark Drew Crosland Guimarães2

1Embrapa Gado de Leite 5Laboratório Nacional Agropecuário, Ministério da Agricultura Pecuária e Abastecimento, Pedro Leopoldo, MG, Brasil
2Programa de Pós-Graduação em Saúde Pública, Departamento de Medicina Preventiva e Social, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil
3Laboratório de Biologia Molecular Aplicada a Micobactérias, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil
4Hospital Regional João Penido, Fundação Hospitalar do Estado de Minas Gerais, Juiz de Fora, MG, Brasil


In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB) patients living in an urban area in Brazil were characterised from 2008-2010 using phenotypic and molecular speciation methods (pncA gene and oxyR pseudogene analysis). Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ) and Stonebrink (SB)-containing media and presented phenotypic and molecular profiles of Mycobacterium tuberculosis, whereas 12 had molecular profiles of M. tuberculosis based on the DNA analysis of formalin-fixed paraffin wax-embedded tissue samples (paraffin blocks). One patient produced two sputum isolates, the first of which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, and the second of which only grew on SB media and presented phenotypic profiles of Mycobacterium bovis. One patient provided a bronchial lavage isolate, which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, but had molecular profiles of M. bovis from paraffin block DNA analysis, and one sample had molecular profiles of M. tuberculosis and M. bovis identified from two distinct paraffin blocks. Moreover, we found a low prevalence (1.6%) of M. bovis among these isolates, which suggests that local health service procedures likely underestimate its real frequency and that it deserves more attention from public health officials.

Received 22 September 2012
Accepted 7 January 2013
Financial support: CNPq (410595/2006-3)/MCT/CNPq/MS-SCTIEDECIT
MRS received a fellowship from the EMBRAPA.
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.


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