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MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 108(3) May 2013
PAGES: 312-320 DOI: 10.1590/0074-0276108032013009 Full paper
Genetic compatibility between Anopheles lesteri from Korea and Anopheles paraliae from Thailand

Kritsana Taai1, Visut Baimai2, Atiporn Saeung1, Sorawat Thongsahuan3, Gi-Sik Min4, Yasushi Otsuka5, Mi-Hyun Park4, Masako Fukuda5,6, Pradya Somboon1, Wej Choochote1,+

1Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand
2Department of Biology and Centre for Vectors and Vector-Borne Diseases, Faculty of Science, Mahidol University, Bangkok, Thailand
3Faculty of Veterinary Science, Prince of Songkla University, Songkhla, Thailand
4Department of Biological Sciences, Inha University, Incheon, South Korea
5Department of Infectious Disease Control, Faculty of Medicine 6Research Promotion Project, Oita University, Hasama, Oita, Japan

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Abstract

To assess differentiation and relationships between Anopheles lesteri and Anopheles paraliae we established three and five iso-female lines of An. lesteri from Korea and An. paraliae from Thailand, respectively. These isolines were used to investigate the genetic relationships between the two taxa by crossing experiments and by comparing DNA sequences of ribosomal DNA second internal transcribed spacer (ITS2) and mitochondrial DNA cytochrome c oxidase subunit I (COI) and subunit II (COII). Results of reciprocal and F1-hybrid crosses between An. lesteri and An. paraliae indicated that they were compatible genetically producing viable progenies and complete synaptic salivary gland polytene chromosomes without inversion loops in all chromosome arms. The pairwise genetic distances of ITS2, COI and COII between these morphological species were 0.040, 0.007-0.017 and 0.008-0.011, respectively. The specific species status of An. paraliae in Thailand and/or other parts of the continent are discussed.

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Received 24 August 2012
Accepted 26 November 2012  
Financial support: The Thailand Research Fund (to WC and AS) (TRF Senior Research Scholar: RTA5480006, TRF Advanced Research Scholar: BRG5380021), Royal Golden Jubilee PhD Program (to WC and KT) (PHD/0297/2551), Faculty of Medicine Endowment Fund, Chiang Mai University
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