PAGES: 773-777 DOI: S0074-02762008000800005 Full paper
Synthesis of limonene ?-amino alcohol derivatives in support of new antileishmanial therapies

Stela R FerrariniI; Cedric S GraebinI; Jones LimbergerII; Rômulo FS CantoI; Daiane O DiasI; Ricardo G da RosaII; Maria de Fátima MadeiraIII; Vera L Eifler-LimaI, +

ILaboratório de Síntese Orgânica Medicinal, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752 sala 705, 90610-000 Porto Alegre, RS, Brasil
IILaboratório de Catálise por Metais de Transição, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752 sala 705, 90610-000 Porto Alegre, RS, Brasil
IIIServiço de Parasitologia Clínica, Instituto de Pesquisa Clínica Evandro Chagas-Fiocruz, Rio de Janeiro, RJ, Brasil


A series of seven limonene β-amino alcohol derivatives has been regioselectively synthesised in moderate to good yields. Two of these compounds were found to be significantly effective against in vitro cultures of the Leishmania (Viannia) braziliensis promastigote form in the micromolar range. The activities found for 3b and 3f were about 100-fold more potent than the standard drug, Pentamidine, in the same test, while limonene did not display any activity. This is the first report of antileishmanial activity by limonene β-amino alcohol derivatives.

Parasitic diseases are the cause of much suffering and death throughout the world, mainly in underdeveloped areas like Africa, Asia and Latin America. In these regions, the economic and social impacts caused by these sicknesses are very high because the parasites infect millions of people. Among these parasites, the Protozoa genera are responsible for the principal sicknesses, and leishmaniasis is one of the strictest parasitic diseases caused by multiple species of Leishmania (WHO 2008). The disease is endemic in tropical regions, affecting over 12 million people in 88 countries and its clinical manifestations can occur in cutaneous, mucocutaneous and visceral forms (Croft & Yeardley 2002, Murray et al. 2005). The main health organisations are concerned about these statistics due to the significant number of cases (Nwaka & Hudson 2006). Despite this, the therapies currently available to treat this illness are deficient and outdated and most present several side-effects. Moreover, the widespread development of resistance by certain strains of leishmaniasis to antimonial compounds contributes to poor health conditions in underdeveloped countries. Chemotherapy of leishmaniasis (Croft & Yeardley 2002, Nwaka & Hudson 2006) consists of using intravenous drugs like antimonial compounds as first-choice drugs and amphotericine B as second-choice. In countries where many resistance cases to the antimonial drugs are reported, this antibiotic is used as a first-choice. Intramuscular Pentamidine is administrated in patients infected with Leishmania panamensis and Leishmania braziliensis. Miltefosine and fluconazole are currently the only effective oral treatments for leishmaniasis. However, all of these treatments have significant drawbacks in terms of route of administration, length of treatment (21-28 days), toxicity and cost, which limit their use in endemic areas (Croft & Yeardley 2002, DNDI 2003, Murray et al. 2005). These arguments by themselves justify the search for new leishmanicidal drugs, which makes the search for new agents a priority for its erradication. In this context, the strategy of following new leads to treat neglected diseases starting from natural sources is widely used by medicinal chemists (Viegas et al. 2006, Newman & Cragg 2007).

Limonene is a main constituent of essential oils of citrus plants, is abundant in nature and is available in both enantiomeric forms, R(+)-limonene 1a and S(-)-limonene 1b (Fig. 1). The literature describes some pharmacological activities attributed to essential oils, including antimalarial (Lopes et al. 1999, Tchoumbougnang et al. 2005), antibacterial, antifungal (Filipowicz et al. 2003, Nostro et al. 2004) and antileishmanial (Ueda-Nakamura et al. 2006) effects. For limonene, some activities have been reported as antiproliferative (Crowell et al. 1991) and antimalarial (Moura et al. 2001). In addition, limonene and some of its derivatives have important roles in the cosmetic industry (Deans & Ritchie 1987, Kim et al. 1995, Beletti et al. 2004). Their use as a building block or a chiral auxiliary in asymmetric synthesis is also well established (Wender et al. 2001, Mehta & Shinde 2003, Srikrishna & Dethe 2003, Tokyatsu et al. 2003). Due to the chemical and biological versatility of limonene, it can be employed as a building block for searching for new hits. Its antimalarial activity in vitro has been demonstrated by some sulphone endoperoxide limonene derivatives (Bachi et al. 1998) and antiproliferative activity was detected for new limonene derivatives with a substituted thiourea moiety (Figueiredo et al. 2006).



In a previous work, we described the selective chemical modulation of the limonene isoprene group with the synthesis of a set of N-alkyl/N-aryl amines through a tandem hydroformilation/reductive amination reaction (Graebin et al. 2008). In the present work, we reported the synthesis of limonene ?-amino alcohols through a regioselective limonene-oxide aminolysis by N-alkyl and N-aryl amines and their in vitro antileishmanial promastigote activity. ?-amino alcohols are an important class of organic compounds due to their common occurrence in nature and because they are versatile building blocks in the synthesis of a wide range of natural and synthetic products. We have used alkylated amines (n-propylamine, allylamine, ethanolamine, benzylamine), diamine (putrescine), cyclic amine (morpholine) and an aromatic one (aniline) in order to generate diversity at the structure, since for all cases founded in the literature, derivatives with a nitrogen and oxygen heteroatom were more active than limonene.



General method to synthesise the amino alcohols - 1-methyl-2-(allylamino)-4-isopropenyl-cyclohexanol 3a: R(+)-limonene oxide 6 (19.7 mmoles), water (0.55 mL) and allylamine (56.4 mmoles) were added to a glass beaker and heated for 24 h under magnetic stirring at 100ºC. The product was purified by acid-base extraction, treated with CaCl2 and dried in a rotator evaporator. Yield = 78%. FTIR (neat, cm-1): 3394, 3082, 2937, 1643, 1454. Mass spectrometry (M/z, rel. abundance in %): 209 (M+, 0.83). 1H-NMR (CDCl3): 1.2 (3H, s), 1.5 (3H, m), 1.7 (2H, s), 1.85 (3H, s), 2.0 (2H, m), 2.2 (2H, s), 2.55 (1H, m), 3.15 (1H, dd, Ja = 15 Hz, Jb = 7.5 Hz), 3.35 (1H, dd, Ja = 15 Hz, Jb = 7.5 Hz), 4.8 (2H, s), 5.1 (1H, m), 5.25 (1H, m), 5.9 (1H, m). 13C-NMR: 22, 25, 26, 30, 34, 38, 51, 61, 72, 108, 116, 138, 148.

1-methyl-2-(n-propylamino)-4-isopropenyl-cyclohexanol 3b - 1H-RMN (CDCl3): 0.9 (t, 3H, J = 7.4 Hz), 1.2 (s, 3H), 1.5 (m, 4H), 1.7 (s, 3H), 1.98 (m, 2H), 2.45 (m, 1H), 2.5 (m, 1H), 2.6 (m, 4H), 4.8 (s, 2H). 13C-RMN 11.7, 21.5, 23.4, 25.5, 34.4, 37.9, 50.0, 61.7, 71.9, 109.2, 148.4.

1-methyl-2-(ethanolamino)-4-isopropenyl-cyclohexanol 3c - 1H-RMN (CDCl3): 1.2 (s, 3H), 1.58 (m, 4H), 1.7 (s, 3H), 1.8 (m 2H), 2.1 (m, 1H), 2.59 (m, 1H), 2.66 (m, 2H), 2.85 (m, 2H), 4.7 (s, 1H), 4.8 (s, 1H). 13C-RMN: 21.5, 25.9, 30.2, 34.7, 37.9, 49.3, 60.9, 61.4, 72.0, 109.5, 148.3.

1-methyl-2-N-(1,4-diaminobutane)-4-isopropenyl-cyclohexanol 3d - 1H-RMN (CDCl3): 1.2 (s, 3H), 1.47 (m, 4H), 1.50 (m, 4H), 1.7 (s, 3H), 1.95 (m, 2H), 2.1 (m, 1H), 2.15 (m, 1H), 2.47 (m, 1H), 2.69 (m, 4H), 4.7 (s, 1H), 4.8 (s, 1H). 13C-RMN: 21.5, 25.9, 27.6, 30.1, 31.0, 34.6, 37.9, 41.7, 47.8, 61.7, 71.9, 109.3, 148.4.

1-methyl-2-(N-morpholino)-4-isopropenyl-cyclohexanol 3e - 1H-RMN (CDCl3): 1.1 (s, 3H), 1.48 (m, 4H), 1.7 (s, 3H), 1.95 (m 1H), 2.0 (m, 2H), 2.4 (m, 5H), 3.6 (m, 4H), 4.7 (s, 1H), 4.8 (s, 1H). 13C-RMN: 22.1, 22.3, 24.4, 24.8, 35.5, 38.8, 51.9, 67.3, 67.4, 72.6, 110.9, 145.3.

1-methyl-2-(N-phenylamino)-4-isopropenyl-cyclohexanol 3f - 1H-RMN (CDCl3): 1.2 (s 3H), 1.7 (s, 3H), 1.62 (m, 1H), 1.93 (m, 2H), 2.1 (m, 1H), 3.49 (m, 1H), 4.7 (s, 2H), 6.9 (m, 3H), 7.1 (m, 2H), 13C-RMN: 21.3, 26.1, 31.3, 34.4, 38.9, 57.6, 72.4, 110.0, 112.6, 147.6, 148.8, 116.9, 118.8.

1-methyl2-(N-benzylamino)-4-isopropenyl-cyclohexanol 3g - 1H-RMN (CDCl3): 1.1 (s, 3), 1.58 (m, 2H), 1.7 (s, 3H), 1.86 (m, 2), 2.15 (m, 1H), 2.5 (m, 1H), 4.8 (s, 2H), 3.67 (m, 1H), 3.85 (m, 1H), 7.27 (s, 5H). 13C-RMN: 21.4, 26.1, 30.0, 34.4, 37.9, 52.1, 61.2, 72.0, 109.3, 126.9, 128.1, 128.5, 140.7, 148.7.

7-allyl-4-isopropenyl-1-methyl-7-azabicycle[4.1.0]-heptane 4 - A solution of 9.58 mmols of PPh3Br2 in acetonitrile was added drop wise over a solution of 9.58 mmols of 3a in an ice bath with magnetic stirring. Then triethylamine (14.2 mmol) was added, and the reaction was stirred for an additional 5 min, after which the reaction mixture was decanted for 24 h. The liquid phase of the mixture was filtered with Celite and the resulting clear orange solution was dried in a rotary evaporator. The solid residue was treated with hexane, the organic layer was dried with a rotatory evaporator and the oily residue of this evaporation was purified by column chromatography, using hexane/ethyl acetate (6:1) as the eluent. Yield = 40%. Mass spectrometry (M/z, rel. abundance in %): 191 (M+, 1.67). 1H-NMR (CDCl3): 1.15 (1H, m), 1.22 (3H, s), 1.5 (2H, m), 1.7 (3H, s), 1.76 (3H, m), 1.85 (1H, m), 2.03 (1H, dd, Ja = 17 Hz, Jb = 4.3 Hz), 3.08 (2H, m), 4.7 (2H, dd, Ja = 17.6 Hz, Jb = 2.1 Hz), 5.1 (1H, dq, Ja = 17.1 Hz, Jb = 2.1 Hz), 5.25 (1H, dq, Ja = 17.1 Hz, Jb = 2.1 Hz), 5.95 (1H, m, J = 4.7 Hz). 13CNMR: 18, 21, 26, 30, 33, 37, 38, 46, 55, 109, 117, 137, 150.

Leishmanicidal assay - This assay was performed as described by Machado et al. (2007). Cultures of L. (Viannia) braziliensis (MCAN/BR/98/R.619) were maintained in Schneider's Drosophila medium (pH 7.2) with foetal bovine serum (10%) at 26ºC and on the fourth day of growth, the culture was sedimented by centrifugation (10 min, 4ºC, 4000 rpm), suspended in 1 mL of the same medium and quantified in a Neubauer chamber. Enough medium was added in order to adjust the parasitic concentration to 2 x 106 promastigotes/mL. The determination of the leishmanicidal activity was performed in 96-well plates. The compounds were tested in triplicate in a concentration gradient from 320-0.156 µg/mL. The parasitic concentration in the wells was of 2 x 105 promastigotes/mL. A negative control was performed with three wells containing only parasites and the incubation medium. The positive control was made with Pentamidine isothionate (320 µg/mL). After 24 h of incubation at 26ºC, 10 µL of each well was diluted in 90 µL of the vital colorant (trypan blue in PBS) and the parasites were quantified in a Neubauer chamber. The data obtained from this quantification were plotted in a graph using Microsoft Excel and the LD50 was extrapolated from the graph as the concentration of the products that inhibited the parasitic growth at 50% of the values of the negative control.

Relative toxicity assay - The cytotoxic effect of the compounds described in this paper was evaluated in murine peritoneal macrophages using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT) in a colourimetric assay as previously reported (Mosmann 1983). Briefly, the cells were isolated from the peritoneal cavity of mice and, after adjusting the cell concentration to 4 x 105 cells/mL of medium, the cells were cultivated on a 96-well microtitre plate with RPMI medium, supplemented with 10% of foetal calf serum and incubated at 37ºC in a humidified atmosphere with 5% CO2. The compounds were added in triplicate to the cell culture at the respective LD50 concentrations. Three wells of the cell culture, without adding any compound, were used as controls. The plate was then incubated in the same conditions as described above for 24 h. Then, 22 µL of an MTT solution (5 mg/mL) was added to each well and the plate was further incubated for 3 h. The enzymatic reaction was stopped with addition of 80 µL of DMSO, the plate was gently shaken at rt for 15 min and the optical density was read in a spectrophotometer (492 nm). The toxicity was expressed as a relative value, using the optical density of the control wells as a reference.



Aminolysis is a classical route for the preparation of ?-amino alcohols from epoxides, and a large excess of amines is typically used, leading to products with moderate or low yields. In this paper, we have employed the R-(+)-limonene oxide 2 stirred with one equivalent of amine (except for aniline, where it was necessary to use 2 equivalents) and three equivalents of water at reflux for 24 h to prepare the ?-amino alcohols 3a-3g (Fig. 2) in moderate to good yields, as highlighted in Table I, according to a previously reported chemical pathway (Chrisman et al. 2001). Three of these molecules are new: 3a, 3c and 3d (Kozhin et al. 1978, Chrisman et al. 2001, Singaram et al. 2002, Steiner et al. 2002). The R-(+)-limonene oxide 2 was chosen as the starting material because it is readily available and inexpensive. Moreover, the synthesis of this epoxide from limonene (Jones et al. 1999) would add an avoidable step to the synthetic pathway. The original aziridine 4 was obtained from 3a in the presence of PPh3Br2 under basic conditions. The compounds were purified and characterised by classical analytic methods.





It is known that, due to conformation of the ring of limonene oxide, the aminolysis is regioselective, leading only to the trans regioisomer (Chrisman et al. 2001, Singaram et al. 2002). The conditions carried out in this work allow for a regioselective ring opening resulting in ?-amino alcohols with a trans conformation as the only or major product, which was attributed by NMR and CG analyses. For example, the 1H-NMR spectrum of 3a demonstrates additional peaks attributed to NH-allyl and to OH groups at ? = 3.25 ppm. For the vicinal amino alcohol 3f, we can detect the additional peaks corresponding to the NH-phenyl unit and the OH group at ? = 3.9 ppm and to the aromatic protons at ? = 6.6 and 7.1 ppm. The selectivity of this reaction can be explained by the conformational differences between cis and trans diastereomers (Steiner et al. 2002). Concerning the trans isomer, the ring opening by the amines occurs mainly on the less hindered side of the epoxide, via a transition state that is thermodynamically more stable in a chair conformation. The boat conformation is not so energetically favored, and the cis epoxide can be recovered at the end of the reaction. The aziridine 4 was obtained from the reaction of 3a with PPh3Br2 and triethylamine (Fig. 2). The 1H-NMR spectra show that the two vinylic protons of the isopropenyl group remain untouched at ? = 4.7 ppm. The three new allylic protons at ? = 5.10, 5.25 and 5.95 ppm and the two methyl groups appear as singlets, such that one is in the isopropenyl portion of the molecule at ? =1.7 ppm and the other one is close to the aziridine cycle at 1.21 ppm.

Leishmanicidal activity was determined and, according to the data highlighted in Table II, among the seven ?-amino alcohols tested, two of these were more active than Pentamidine (48.5 ± 28.7 µM) and five were more active than limonene 1a. In fact, the (R)-limonene 1a was inactive in the test (876.2 ± 216 µM), showing that this terpene, when isolated, is not active against promastigotes of L. braziliensis. The derivatives n-propyl 3f (0.408 ± 0.02) and phenyl 3b (0.71 ± 0.095 µM) exhibited more powerful activities. The lipophilic character of the substituents seems to be required in order to interact with the parasite. We can clearly observe this, since, when the activity of the compounds decreases, the polarity increases. The ?-amino alcohols 3f and 3b bearing the hydrophobic substituents phenyl and n-propyl groups were more active (0.408 ± 0.01 µM and 0.71 ± 0.095 µM, respectively), while compound 3a carrying the allyl moiety presented approximately the same potency of Pentamidine (76.5 ± 13.9 µM). The replacement of the lipophilic aliphatic amines by more polar amines resulted in a dramatic decrease in activity as well. The three less active derivatives were 3d with the putrescine chain, 3e with the morpholine ring and 3c with ethanolamine, showing no activity at the highest concentration used in the test.



The substituent volume is another important aspect, since the compound 3g bearing a benzylamine moiety (830.7 ± 185 µM) was much less active than the ?-amino alcohol 3f carrying the aniline (0.408 ± 0.01 µM). It was also observed that the allylaziridine 4 presented low performance at the same test (510.00 ± 92.5 µM). Taking into account the result obtained with 3a, the conformational modifications on the cyclohexane ring of 4 caused by the additional aziridine nucleus, which forms a new bicyclic strained system, can be responsible for the decrease in activity. Future investigations must be performed in order to clarify this lack of activity.

The results obtained with this work are preliminary and can neither establish a Structure-Activity Relationship study of compounds nor speculate about the mechanism of action. However, they characterise the introduction of a heteroatom such as nitrogen in the limonene core, showing that it leads to compounds with promising leishmanicidal activity.

The toxicity assay results, expressed in Table II, show that compounds 3a, 3b and 3f presented low toxicity when compared to the control cell cultures. These three compounds have lower toxicity values than the standard drug used in the leishmanicidal assay, Pentamidine (35.51 ± 2.52 %) and the terpene R-(+)-limonene 1a (14.36 ± 2.32 %), while compounds 3c, 3g and 4 showed moderated toxicity values. Due to their high LD50 values, compounds 3d and 3e were not employed in this assay.

In conclusion, we have demonstrated the in vitro activity of a new class of ?-amino alcohol derivative using R(+)-limonene as a building block against the L. braziliensis promastigote. The results obtained in this series demonstrate that limonene is a good scaffold for the design and synthesis of new ?-amino alcohols intended to search for new hits for this parasite. The two most active compounds in the leishmanicidal assay (3b and 3f) showed low in vitro toxicity values against a murine macrophage culture. The results are preliminary, but they provide a starting point to design other modifications from the limonene architecture in order to guide further structure activity-relationship studies. The use of an economical and largely available natural product to generate biologically active compounds requiring few synthetic steps is an attractive strategy to discover new lead candidates. As such, studies are currently underway to investigate the mechanism of action for the antileishmanial activity observed here, and these findings will be reported elsewhere.



To Dr. Hugo Verli (UFRGS), for facilitating the first contact between our research groups.



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Received 24 June 2008
Accepted 11 November 2008
Financial support: CAPES/MEC, CNPq, MCT/CNPq 02/2006 _ Universal (Proc. 483674/2006-0).

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