MEM INST OSWALDO CRUZ, RIO DE JANEIRO, Vol. 112 | 2017
PAGES: DOI: 10.1590/0074-02760170171 Short communication
Potential application of rLc36 protein for diagnosis of canine visceral leishmaniasis

Camila Tita Nogueira1, Mayara Lúcia Del Cistia2, Ana Carolina Urbaczek3, Márcia MG Jusi4, Angela Maria Arenas Velásquez1, Rosângela Zacarias Machado4, Henrique Ferreira5, Flávio Henrique-Silva6, Hélio Langoni7, Paulo Inácio da Costa2, Márcia AS Graminha1,2,+

1Universidade Estadual Paulista, Instituto de Química, Campus de Araraquara, Araraquara, SP, Brasil
2Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, Campus de Araraquara, Araraquara, SP, Brasil
3Universidade de São Paulo, Instituto de Química, Campus de São Carlos, São Carlos, SP, Brasil
4Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Campus de Jaboticabal, Jaboticabal, SP, Brasil
5Universidade Estadual Paulista, Instituto de Biociências, Campus de Rio Claro, Rio Claro, SP, Brasil
6Universidade Federal de São Carlos, Departamento de Genética e Evolução, São Carlos, SP, Brasil
7Universidade Estadual Paulista, Faculdade de Medicina Veterinária e de Zootecnia, Campus de Botucatu, Botucatu, SP, Brasil

Abstract

Visceral leishmaniasis (VL) is fatal if left untreated. Infected dogs are important reservoirs of the disease, and thus specific identification of infected animals is very important. Several diagnostic tests have been developed for canine VL (CVL); however, these tests show varied specificity and sensitivity. The present study describes the recombinant protein rLc36, expressed by Leishmania infantum, as potential antigen for more sensitive and specific diagnosis of CVL based on an immunoenzymatic assay. The concentration of 1.0 μg/mL of rLc36 enabled differentiation of positive and negative sera and showed a sensitivity of 85% and specificity of 71% (with 95% confidence), with an accuracy of 76%.

Financial support: FAPESP (grant #2010/26732-2), CAPES, PADC.
CTN was supported by the FAPESP fellowship (#2011/06995-9) and MLDC by the FAPESP (#2011/15508-4) and CAPES fellowships.
CTN and MLDC contributed equally to the study.
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 27 April 2017
Accepted 24 October 2017

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