MEM INST OSWALDO CRUZ, RIO DE JANEIRO, Vol. 113 | 2018
PAGES: DOI: 10.1590/0074-02760170250 Short communication
Leishmania (Viannia) braziliensis isolated from the saliva of patients in a cutaneous leishmaniasis-endemic area of northeastern Brazil

Maria Edileuza Felinto de Brito1,+, Ericka Lima Almeida1, Angela Cristina Rapela Medeiros2, Roberto Pereira Werkhäuser1, Joanna Lucia de Almeida Alexandre1, Bruna Santos Lima Figueiredo Sá1, Eduardo Henrique Gomes Rodrigues1, Sinval Pinto Brandão-Filho1

1Fundação Oswaldo Cruz-Fiocruz, Instituto Aggeu Magalhães, Departamento de Imunologia, Recife, PE, Brasil
2Universidade de Pernambuco, Recife, PE, Brasil

Abstract

Several studies have described the use of non-invasive collection methods, mostly based on the detection of parasite DNA, for diagnosis. However, no Leishmania specimens have been isolated from saliva. Here, we report the first isolation of Leishmania braziliensis from the saliva of humans with cutaneous leishmaniasis but without lesions on their mucosa. The isolates were obtained from salivary fluid inoculated in hamsters and were tested by multilocus enzyme electrophoresis. Seven samples from 43 patients suspected of having the disease were identified for in vivo culture. These findings suggest that saliva is a clinical sample that allows the isolation of Leishmania sp.

+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 10 July 2017
Accepted 1 December 2017

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