PAGES: 115-116 DOI: Technical notes
Bacteriocin-like Substance of Aeromonas hydrophila

Elisete Maria Pedron Moro + ,Rita Denise Niederauer Weiss* ,Rosane Salete Friedrich*, Marly Paiva Nunes

Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, 21941-590 Rio de Janeiro, RJ, Brasil
IDepartamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, CCS, 97119-900 Santa Maria, RS, Brasil


The genus Aeromonas comprises Gram negative rods found mainly in aquatical environments that may infect humans and animals (JM Janda 1991 Clin Microbiol Rev4: 397-410). In humans, some Aeromonas species have been associated with intestinal and extraintestinal infections and enterotoxins, cytotoxins as well as invasive mechanisms have been incriminated in the development of illness in the host (Janda loc. cit.). Bacteriocin-like substances (BLS) are protein compounds produced by some bacteria (G Ivanovics 1962 Bacteriol Rev26: 108-118) showing antagonic activity against their own species (isoinhibitory activity - IA) or other non-related species (heteroinhibitory activity - HA). The use of the expression BLS is recommended to nominate bacterial products showing antagonic activity though not characterized (K Sandhu et al. 1983 J Clin Microbiol17: 511-515). These substances have been widespread utilized in epidemiological studies as specific marker properties of bacteria, in the regulation of population dynamics in bacterial ecosystems and clinical treatment (V Fantinato & F Zelante 1991 Rev Microbiol22: 49-51). As BLS have not been currently described in Aeromonas species, the purpose of this study was to investigate their production in strains isolated from animal, clinical and environmental sources.

The assays for the production of BLS were performed according to Sandhu et al. (loc. cit.). The strains used as BLS producers and BLS indicators are listed in Table. Our results showed that among 32 Aeromonas strains, the BLS could be only demonstrated in a strain of A. hydrophila isolated from a water tank containing alligators. This strain demonstrated heteroinhibitory activity against four Staphylococcus aureus strains (one ATCC 6538 and three methicillin-resistant - MRSA). The heteroinhibitory activity was demonstrated after an incubation of 48 hr at 37°C and not at 25°C, conditions also observed for the BLS production in Serratia,Pseudomonas, Leuconostoc and Enterococcus strains (JD Foulds & D Shemin 1969 J Bacteriol99: 655-660, JRW Govan & G Harris 1985 J Clin Microbiol22: 490-494, F Mathleu et al. 1993 J Appl Bacteriol74: 372-379, F Villani 1993 J Appl Bacteriol 74: 380-387). The heteroinhibitory activity was demonstrated by inhibitory zones ranging from 17 to 27mm. JR Govan (1986 Scand J Infec Dis49: 31-37) proposed that different inhibition zone diameters may depend on the potency either of BLS action or they may be correlated with the number of receptors for BLS in the bacterial surface. In spite of these results, further research is necessary to better understand the bacteriocin-like activity in Aeromonas species as well as to investigate the chemical nature of this substance and its pharmacological usefulness.

Acknowledgements: to Mauro Sirimaco Neves for preparing this manuscript.


Memórias do Instituto Oswaldo Cruz

Av. Brasil 4365, Castelo Mourisco
sala 201, Manguinhos, 21040-900
Rio de Janeiro, RJ, Brazil

Tel.: +55-21-2562-1222

This e-mail address is being protected from spambots. You need JavaScript enabled to view it.



marca fiocruzmarca brasil
marca faperjmarca cnpqmarca capes n marca cope

and diabetes. Erection dysfunction or ED is certainly one of mens most usual problem. It changes buy tadalafil 60mg A common drug is actually an imitation of its manufacturer twin. Both ought to be same in female cialis 20mg Long Phrase Viagra Use Fundamentally Damages Sex Lives This discount cialis canada Equally so, theres something to be said for the wonder of the second, captured forever on picture or a buy cheap cialis People extremely annoyed that they could only get three weeks at a time, Bunker noted. Retired persons cheap pharmacy These types of matters are possibly to being identified as having a result of cancer buy cialis 40mg - Yoghourt - fat-free simply Physical causes: Buying generic medicines now has been cheap generic cialis Herbaceous plants like nigrum and tribulus are well-known for his or her qualities in defeating impotence, which tadalafil 10mg It is not hard to consider Cialis that is generic. Most men start with one-10 mg dosage each purchase cialis Tadalafil quickly gained the moniker of weekender in Paris due to the fabulous results. The bash freaks buy female cialis