PAGES: 605-610 DOI: Full paper
Electrocardiographic Findings in Mexican Chagasic Subjects Living in High and Low Endemic Regions ofTrypanosoma cruzi Infection

Francisca Sosa-JuradoI, Miguel Mazariego-ArandaII, Nidia Hernández-Becerril, Verónica Garza- Murillo, Manuel Cárdenas, Pedro A Reyes, Kenji HirayamaIII, Victor M Monteón +

Instituto Nacional Cardiología "I. Chávez", Juan Badiano 1, Col Sección XVI, México DF 14080
IHospital de Especialidades Centro Médico Nacional "Manuel Ávila Camacho" IMSS, Puebla, México
IIFacultad de Ciencias Químicas, Campus IV, Tapachula Chiapas, México
IIIInstitute of Tropical Medicine, Nagasaki University, Japan


In México the first human chronic chagasic case was recognized in 1940. In spite of an increasing number of cases detected since that time, Chagas disease in México has been poorly documented. In the present work we studied 617 volunteers subjects living in high and low endemic regions of Trypanosoma cruzi infection with seroprevalence of 22% and 4% respectively. Hemoculture performed in those seropositive subjects failed to demonstrate circulating parasites, however polymerase chain reaction identified up to 60% of them as positives. A higher level of anti-T. cruzi antibodies was observed in seropositive residents in high endemic region, in spite of similar parasite persistence (p 0.05). On standard 12 leads electrocardiogram (ECG) 20% to 22% seropositive individuals from either region showed riht bundle branch block or ventricular extrasystoles which were more prevalent in seropositive than in seronegative individuals (p 0.05). In conclusion, the frequency or type of ECG abnormality was influenced by serologic status but not by endemicity or parasite persistence. Furthermore, Mexican indeterminate patients have a similar ECG pattern to those reported in South Amrica.

Chagas disease is an important public health disorder in Latin America. This infectious disease is caused byTrypanosoma cruzi and transmitted by Triatominae insects in most cases.

Data from the World Health Organization state that approximately 90 million people in Latin American countries are exposed to this infection (WHO 1991).

México has an ample array of Triatominae insects, 31 species have been recognized, and it has been found that most of them are naturally infected with T. cruzi (Zarate & Zarate 1980, 1985, Vidal-Acosta et al. 2000).

Epidemiological surveys carried out in different regions of the country have revealed an heterogeneous distribution of the infection (Tay et al. 1980, Salazar et al. 1988, Velasco-Castrejón 1992). Endemic zones have been identified mainly in the coastal and southern regions of México with high seropositivity in rural villages, as well as cases of chronic heart disease (Ruegsegger et al. 1993, Mendoza et al. 1995, Zavála-Castro et al. 1995 Monteón et al. 1996, Hernández-Becerril et al. 2001, Mazariego-Aranda et al. 2001). More recently moderate to low seropositivity has been reported in the central part of México (Pérez-Fuente et al. 1998, Rangel-Flores et al. 2001).

The outcome of the disease and progression from indeterminate form of chronic phase to symptomatic in infected people is not completely understood. It has been suggested that several of the role playing factors are: genetic host background, parasite strain, and reinfection (Mendoza et al. 1995, Zavála-Castro et al. 1995, Montamant et al. 1996, Fernández-Mestre et al. 1998, Bustamante et al. 2002).

Chagas disease in México has not been fully do-cumented and scarce reports exist on the indeterminate form of chronic phase. To study this indeterminate chronic phase is an important step towards the systematic study of the disease. In the present work we studied volunteers subjects from two communities with high and low seroprevalence for T. cruzi infection.



Study area - The study was done in nine rural villages (municipality Palmar de Bravo) located in the highlands, central part of México, Puebla state (2 150-2 500 m above sea level), range of temperature from 10°C to 26°C with 7 200 inhabitants, the second area encompasses three communities in the lowlands, located in the southern part of México, Chiapas state (600 m above sea level), range of temperature 23°C to 35°C, with 1 500 inhabitants (Fig. 1)

Studied population - In Puebla state, 390 volunteers (Palmar de Bravo municipality) ranging from 15 to 65 years old participated in the study, the mean age was 36.6 ± 12.3 years old, and 93% were female. The excessive proportion of female individuals and poor participation responded to cultural behavior, in general male individuals refused to participate in this survey.

In Chiapas state, 227 volunteers from the Lacandon Rainforest ranging from 6 to 73 years old participated in the study, the mean age was 25 ± 18.6, and 63% were females. In this area it was possible to enroll children from local elementary school, obviously under parents approval.

Serology for anti- T. cruzi antibodies, hemoculture, polymerase chain reaction (PCR) specific for T. cruzi was performed in all participants. Electrocardiograms (ECGs) were performed in seropositives volunteers and age matched seronegatives in both areas.

Ethical considerations - This work was done in accordance to "Reglamento de la Ley General de Salud en Materia de Investigación" which stated forearm venipucture as a minimal risk procedure and requires oral consent when illiterate participants are invited to a clinical or epidemiological study. When feasible a written informed consent letter was used. The Ethics Committee of the Institute (Instituto Nacional Cardiología "I. Chávez") approved the study protocol.

Serological test - Indirect immunofluorescence (IIF) detected total IgG anti- T .cruzi antibodies and enzyme linked immunosorbent assay (ELISA) as described previously with minor modifications. In brief, IIF was performed on T. cruzi epimastigote phase, fixed on a microscope slide. Human serum was diluted 1:32 and fluorescein conjugated goat anti-human IgG was used at 1:100 dilution. Positive and negative controls were always included, and the slide was read under epifluorescence microscope (Hernández-Becerril et al. 2001).

ELISA was done using polystyrene plates coated with 10 µg/ml of protein of T. cruzi (Ninoa Mexican strain) extract in alkaline-buffered solution. Human serum was diluted at 1:200 and anti-human IgG-peroxidase conjugate was used at 1:15 000 dilution. The reaction was revealed by addition of O-phenilen-diamine and read at 490 nm in automatic ELISA reader (Hernández-Becerril et al. 2001).

Cut off value was set as follows: sera from 30 healthy seronegative people were pooled and individually tested. Mean OD ratios obtained between individual negative sample and pooled negative sample was analyzed for their distribution. The mean OD ratio of seronegative healthy individuals plus 3 SD was fixed to set the cut off. All healthy individuals had values classified as negative.

ECG - In all participants a standard 12 leads ECG was recorded at a paper speed of 25 mm/s with a portable Fukuda Denshi Model FX-2111 electrocardiograph (Fukuda Co.). The tracing was blind analyzed in-dependently by two cardiologists. The criteria for the ECG interpretation were those utilized by the Department of Electrocardiography and Electrophysiology of the Instituto Nacional de Cardiología based on the deductive method of ECG interpretation (De Michelli 1993).

Special attention was given to the presence of ar-rythmias, A-V heart block, bundle branch blocks (HBBB), fascicular blocks (Cárdenas 1976), atrial and ventricular hypertrophy (Sodi-Pallareset al. 1964), and repolarization abnormalities with change in the ventricular gradient (Cabrera 1958).

Hemoculture - We followed the technique previously described (Luz et al. 1994) with an adjustment. Fifteen milliliters of heparinized blood was spun down at 4 000 g for 10 min at 4°C. Plasma was removed and sedimented cells were washed with liver infusion tryptose mediun (LIT) and spun again. The supernatant was removed and settled cells were dived into four tubes with equal amount of LIT supplemented with 10% of fetal calf serum and incubated at 28°C for four months. Monthly evaluation of cultures was performed taking out 10-20 µl aliquots from each tube and observing it under light microscope (400X).

PCR for T. cruzi detection - From 1 to 2 ml of blood sample from each individuals DNA extraction was performed as previously reported (Monteón-Padilla et al. 1999). Lysis buffer was added to each sample (SDS final concentration of 1% and proteinase K at 0.2 mg/ml). The mixture was incubated for 3 h at 42°C and extracted twice with phenol/cloroform. Subsequently, sodium acetate (final concentration 0.3 M) and ethanol 95% added for DNA precipitation. DNA pellet was resuspended in 20 µl of sterile water.

Amplification reaction was performed in 50 µl volume containing DNA sample (1 µl), 300 ng of minicircle specific primers KNS1 and KNS2, 0.2 M of each nucleotide, with 2.5 U of Taq polymerase. The reaction was subject to 35 cycles, each cycle 94°C for 1 min, 56°C for 1 min, and 72°C for 1 min. The amplified product was run in 2% agarose, ethidium bromide stained, and visualized under UV illumination. Positive and negative controls were always included for each run. The sensitivity detection of the assay is 0.25 fg of total T. cruzi DNA (Monteón et al. 1994).

Statistical analysis - The descriptive section included mean and standard deviation. The inferential section was supported by the Mann-Whitney test and Chi-square using GraphPad Prism tm version 2.0.



From 390 volunteers studied in the highland (Puebla state), located between 2 150 to 2 500 m above sea level, 15 were seropositives by two serological test (4%). People from these communities are in general poor and malnourish. Houses are made of wood or occasionally brick. The vector recognized was Triatoma barberi. Hemoculture performed in those seropositive subjects failed to demonstrate circulating parasites, but PCR disclosed 60% of them as T. cruzi DNA carriers (Table IFig. 2).

People lived in the lowland (Chiapas state), in the Lacandon Rainforest with a very precarious housing condition; up to 73% of their houses are made of palms leaves or other natural materials. The bug's eggs are easily carried on this material and the vector becomes domiciliated. The vector recognized was Rhodnius prolixus. Seroprevalence was 22% (50 out 227 volunteers). Hemoculture evidence for circulating T. cruzi was also negative but, again PCR showed 43% of positive individuals among seropositive population (Table IFig. 2).

Level of IgG anti-T. cruzi antibodies between subjects from highlands and lowlands had a clear difference in antibody titer (Fig. 3). Individuals resident in endemic place (lowland: seroprevalence 22%) showed higher level of anti-T. cruzi antibodies than resident from low endemic region (highlands: seroprevalence 4%) with statistical significance p < 0.05 (Mann-Whitney test) but not association between level of antibodies and abnormal ECGs was observed (data not shown).

Ten out of 15 seropositive individuals and 15 seronegative age matched control from the highlands (Puebla state) and 35 out of 50 seropositives and 19 seronegative age matched control from lowlands (Chiapas state) accepted ECG examination.

Only two seropositive subjects from highlands had abnormal ECGs, both with incomplete RBBB. The proportion of seropositive individuals presenting ECG alterations represented 20% (Table II). One more presented non-specific T wave changes (data not shown). From the seronegative group none presented BBB (Table II), only one showed prolonged (0-06 mseg) QT segment (data not shown).

In the lowlands eight out of 35 seropositives (22%) had abnormal ECG, four of them presented incomplete RBBB and two complete RBBB, one showed abnormal left ventricular repolarization, and another one presented arrhytmia (ventricular extrasystole). In the age matched seronegative group only two out 19 (11%) had abnormal ECG with incomplete RBBB (Table II). The association between positive serology and abnormal ECGs was statistically significant in both groups (Chi-square p < 0.05).

Finally, there was no difference between place of residence and type or frequency of ECGs alterations.

Table III shows that regardless of age of seropositive individuals had no association with abnormal ECG, same frequency of altered ECG was found either young or old people. In the age group between 20 to 40 years old, four out of 19 seropositive individuals had abnormal ECGs, representing 21% and for seropositive individuals above 40 years old a discrete increase was observed: six out of 23, representing 26%, without any statistical significance. The same was true when compared mean age of seropositives with normal or abnormal ECG.



The results of this study extends and corroborates the heterogeneous distribution of T. cruzi infection in México (Salazar et al. 1988, Velasco-Castrejón et al. 1992, Ruegsegger et al. 1993, Mendoza et al. 1995, Zavála-Casrtro et al. 1995, Mazariego-Aranda et al. 2001, Rangel-Flores et al. 200), linked to geographic differences, housing conditions, as well as the kind of vector.

In this study higher seroprevalence was observed in the Lacandon Rainforest housing and the presence of an aggressive vector (R. prolixus), in addition to ignorance triatomine-borne infectious and no vector control, enhanced transmission and potential risk to reinfection. Unfortunately trypanosomiasis is not yet considered as priority in the Mexican Health Programs. This is the reason why transmission continues high in some areas of México.

Although both studied regions share several risk factors, including similar malnourish prevalence, in the highland the vector was different (T. barberi) and localized outside of living houses. Unfortunately cultural patterns of inhabitants did not allow to measure infestation index in order to compare them, in addition to the poor interest to participate in this survey.

Detection of circulating parasite is the best proof of infection. This is a difficult task in the chronic phase. We applied hemoculture technique to search for circulating T. cruzi with negative results due to small amount of blood was used to performed it as volunteers refused to give more than 15 ml of blood, even though parasitemia was revealed by PCR in 43% and 60% of seropositive individuals from high and low endemic place respectively.

Indicating a very low parasite-circulating load in these chronic infected subjects. Despite, differences in infection rate between high and low endemic regions, parasitemia detected by PCR was comparable for both groups (Table I).

In our study we found that people living in high endemic areas for T. cruzi infection, showed higher anti- T. cruziantibodies than people from low endemic areas (p < 0.05). This finding may suggests that reinfection could take place in high endemic region enhancing high level of specific antibodies. Although, in human beings it is difficult to prove that reinoculation is taking place, an animal model has been tested and measured indirectly by the antibody levels, showing that during the chronic phase higher anti-T. cruzi antibody levels is presented in reinfected dogs than in those infected only once (p < 0.05) (Machado et al. 2001). Then, high anti-T. cruzi antibodies level in people from endemic areas may be a reflex of reinfection besides parasite persistence that is because by PCR, it was possible to amplified parasite DNA in both people from high and low endemic regions.

It is also known that chronic chagasic cardiomyopathy patients display high anti-T. cruzi antibodies even in those infected cases that lived in endemic zones for only short time of their life (Cerban et al. 1993, Umezawa et al. 1996, Hernández-Becerril et al. 2001). This conflicting point deserves further analysis to explain why antibody levels could be different between resident from high and low endemic regions in spite of parasite persistence in both groups.

In regard to ECG changes, we found mainly conduction defects such as RBBB, the most prevalent finding in seropositive individuals, four times more frequent than among seronegative ones, with statistical significance (p < 0.05). Non-specific T wave and S-T segment changes were found in some cases without correlation with seropositivity. No other ECG changes were recorded

Although, nonspecific ECG abnormalities have been previously reported in healthy population, the most prevalent are non-specific T wave and S-T segment represent 8.6 subjects per 1 000, RBBB 1.6 per 1 000 and LBBB 0.2 per 1 000 (Averill & Lamb 1960). In contrast, among seropositive subjects correlation between abnormal ECG tracings and positivity for T. cruzi was found.

The prevalence rate of abnormal ECGs in seropositive subjects was not influenced by either region or rate of infection or reinfection. In both low-endemic and high endemic regions, abnormal ECG tracing was around 20% in the seropositive population. Although, it has been suggested that reinfection may play a role in the evolution of Chagas disease (Avila et al. 1987), in our data, we found the same frequency of ECGs alteration for both groups from low and high endemic areas.

Previous longitudinal studies carried out in the chronic phase in Brazilian individuals have shown electro-cardiographic changes ranging from 23% to 38% during long-term follow-up (Dias 1985, Coura et al. 1985). While in a transversal study carried out in two different geo-graphic areas in Ecuador abnormal ECG findings in seropositive subjects was between 15% to 40% (Kawabata et al. 1987). Present results showed ECGs abnormalities in up to 22% in the seropositive individuals from either high or low endemic areas.

This data coincides with that reported in South Am-erican literature. However, it has to be emphasized that ECG abnormalities taken into account in this work were only those compatible with rhythm and conduction defects a recognized early feature for heart involvement in chronically infected chagasic people. Although, statistical significance was obtained for seropositive subjects with ECG alterations, definitive conduction defect associated with positive serology was present only in 16% of them. Similar low percentage was also reported in a recently published paper studying Brazilian chronic infected subjects, those who developed conduction defects like RBBB, LBBB, atriventricular blockage, or all of them (Ianni et al. 2001). In general, the indeterminate form of Chagas disease recognized in our population is similar to those reported in South America.

Subtle changes have been detected in Brazilian patients using nuclear angiographies, cardiac cathe-terization and even endomyocardial biopsy (Mady & Galoano 1979, Pereira-Barreto et al. 1986, Marin-Neto et al. 1988), these are invasive procedures which are ethically questionable because these alterations are generally not intense enough to lead to deterioration of myocardial function, furthermore they are not generally available in field studies. Non invasive techniques such as echo-cardiography represent a versatile method in the evaluation of the heart, and it has a good correlation with invasive methods in the determination of ejection fraction. Evaluation of indeterminate chagasic patients by echocardiography and its variant, the so called Doppler Tissue Imaging showed right ventricular functional changes statistically different from a comparison population (Lins et al. 2002).

Although no inference is acceptable in regards to our observations, and echocardiography was not available for us in this field study, the presence of abnormal conduction of the heart electric impulse detectable by ECG in a fifth of tested people, points out that non overt clinical heart disease does exist and may be a progressive condition develops in susceptible cases. Now it is necessary to carry out long-term-follow-up studies to support these preliminary findings.

In conclusion the present work shows a significant occurrence of human Chagas disease in some parts of México, affecting the general population and producing heart lesions detectable by ECG. The rate of abnormal ECGs in seropositives was not influenced by the grade of endemic, however anti-T. cruzi antibody levels was higher in people living in high endemic than in low endemic zone in spite of parasite persistence in both. Vector control actions and medical care to infected individuals should be encouraged in such areas.



To Silvia Monteón for carefully reading of the manuscript.



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+Corresponding author. Fax: +52-5573.0994. E-mail:  This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 12 February 2003
Accepted 18 June 2003

Research supported by a grant from Instituto Nacional Cardiología I. Chávez (México), a grant-in-Aid for Scientific Research on International Cooperation from Ministry of Education, Science and Culture, Japan (12376001), and a grant from the Japan-US Cooperative Medical Science Program (1999-2002).


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