MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 97(5) July 2002
PAGES: 655-656 DOI: Full paper
A New Species of Thelastomathidae (Nematoda) a Parasite of Neocurtilla claraziana Saussure (Orthoptera, Gryllotalpidae) in Argentina

Nora B Camino +, Bárbara Maiztegui

Centro de Estudios Parasitológicos y de Vectores, Calle 2 N° 584, 1900 La Plata, Argentina

Abstract

Gryllophila cephalobulata n. sp. (Nematoda, Thelastomatidae) a parasite of the mole cricket Neocurtilla claraziana (Orthoptera, Gryllotalpidae) isolated in Buenos Aires Province, is described and illustrated. It is characterized by cuticle annulated all along the length of the body; the first ring has 4 lobules, the second one has 14 lobules, the others rings are simple, the stoma is short and has 4 small teeth, the genital papillae are arranged in 5 pairs, of which 3 pairs are preanal and 2 pairs are postanal. The tail appendage of the male is long and filiform.

The genus Gryllophila was proposed by Basir in 1942, with the type species G. gryllophila, having been recovered from a mole cricket. Sergiev (1923) described another nematode from Gryllotalpa in Russia, Thelastomum skrjabini; it appears to be identical to G. gryllophila, so the type species of this genus became Gryllophila skrjabini (Sergiev, 1923) n. comb. Serrano Sanchez (1947) described in Spain the species Neyraiella neyrae, a parasite of Gryllotalpa, but she erroneously observed 2 spicules in the male, and it is synonyms with G. skrjabini. This single species of the genus was found in Russia, India, France, Spain, and Brazil. In this paper we report a second species, G. cephalobulata n. sp., found in the mole cricket Neocurtilla claraziana Saussure in Argentina.

 

MATERIALS AND METHODS

Adults of N. claraziana Saussure from Gorina, La Plata, Buenos Aires Province, Argentina, were collected by hand and then placed in individual vials. Using Poinar's (1975) techniques, the insects were kept at 5°C for 10 min, and then dissected in Petri dishes filled with distilled water under a stereoscope microscope. The nematodes were killed by placing them in distilled water at 60°C for 2 min. They were removed to 50% TAF solution in water for 48 h, then into pure TAF. The nematodes were transferred from the fixative to glycerol for slow evaporation of it and clearing of the parasites. Fixed specimens were used for drawings and measurements with the aid of a lucida camera mounted on a Zeiss compound microscope. All measurements are in µm, with ranges in parenthesis.

 

RESULTS

Gryllophila cephalobulata n. sp. (Fig. 1 A-G)

Description: small nematodes, males much smaller than females. Mouth opening sub-triangular surrounded by 8 lip papillae. Cuticle annulated all along the body, the first ring has 4 lobules, the second one has 14 lobules, the other rings are simple. The stoma is short and has 4 small teeth. Oesophagus consisting of a corpus, an isthmus and a valvated basal bulb. Excretory pore is far behind the base of the oesophagus. Vulva protruding and situated in the posterior third of the body. Vagina long and cylindrical. Didelphic. Egg oval, with smooth shell, covering with a membrane uterine. Tail appendage of female short and conical. Male with a single spicule. The genital papillae arranged in 5 pairs, of which 3 pairs are preanal and 2 pairs are postanal. Tail appendage of the male is long and filiform.

Male (n = 12) body length: 907 µm (845-980); width of the head at level of the mouth: 10.2 µm (9.4-12); oesophagus length: 89.3 µm (85-92.4); distance from anterior end to excretory pore: 103.4 µm (97.8-112); greatest width of body: 44.6 µm (42.2-52); spicules length: 19.5 µm (18.8-21.2); tail appendage length: 61.1 µm (59.6-65.4).

Female (n = 15) body length: 1610 µm (1430-1800); width of the head at level of the mouth: 21.1 µm (20.4-23.6); oesophagus length: 289 µm (260-296.5); distance from anterior end to excretory pore: 306 µm (302.2-321,9); width of body at level of vulva: 101.1 µm (100-106.4); greatest width of body: 98.7 µm (96-99.2); vagina length: 124.5 µm (122-126.6); V: 77 % (76-78.2); length and width of eggs: 25 µm (24.3-25.8) x 11.7 µm (11-12.2); tail appendage 
length: 199.7 µm (196-200.2).

Type host: adults of N. claraziana Saussure (Orthoptera: Gryllotalpidae).

Type locality: Gorina, La Plata, Buenos Aires, Argentina

Site in host: intestine

Type material: deposited in the Helminthological collection of Museo de Ciencias Naturales de La Plata, no. 4858.

 

DISCUSSION

G. cephalobulata n. sp. is very similar to G. skrjabini in having the body annulated throughout its length, the stoma short and cylindrical, the excretory pore is posterior to the base of the oesophagus, the intestine is dilated anteriorly, the vulva is in the posterior third of the body, the eggs are inclosed in a tubular structure into the uteri, the male has one spicule.

G. skrjabini can be separated from our new species in having the first and second rings of the cuticle without lobules, the stoma has no teeth, the vulva is not protruding, the male with 3 pairs of genital papillae, of which 1 pair is preanal and 2 pairs are postanal, and the tail appendage of the male is short.

 

REFERENCES

Basir MA 1942. Nematodes parasitic in GryllotalpaRec Indian Mus 44: 95-106.

Poinar Jr GO 1975. Entomogenous Nematodes. A Manual and Host List of Insect-nematode Associations, EJ Brill, Leiden, Germany, 317 pp.

Sergiev PG 1923. Two new nematodes from the intestine of Gryllotalpa vulgaris. Rapport de 21° Seance de la Commission pour l'Étude de la Faune Helminthologique de Russie. Trans State Inst Exp Vet Sci Moscow 1: 183-190.

Serrano Sanchez A 1947. Nematodes parasitos intestinales de los artropodos en España. Rev Iberica Parasitol 7: 279-332.

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