MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 96(5) July 2001
PAGES: 719-721 DOI: Short communication
Sequential Histological Changes in Biomphalaria glabrata during the Course of Schistosoma mansoni Infection

Queli Teixeira Lemos, Zilton A Andrade +

Laboratório de Patologia Experimental, Centro de Pesquisas Gonçalo Moniz-Fiocruz, Rua Valdemar Falcão 121, 40295-001 Salvador, BA, Brasil

Abstract

Biomphalaria glabrata, highly susceptible to Schistosoma mansoni, were seen to shed less and less cercariae along the time of infection. Histological examination kept a close correlation with this changing pattern of cercarial shedding, turning an initial picture of no-reaction (tolerance) gradually into one of hemocyte proliferation with formation of focal encapsulating lesions around disintegrating sporocysts and cercariae, a change that became disseminated toward the 142nd day post miracidial exposure. Findings were suggestive of a gradual installation of acquired immunity in snails infected with S. mansoni.

The evidences of susceptibility or resistance to Schistosoma mansoni infection presented by different species ofBiomphalaria snails may vary considerably (Newton 1952, Pan 1965, Souza et al. 1995). Such variability can even be observed in snails belonging to a same species, from different geographic areas (Correa et al. 1979). The capacity of an infected snail to shed cercariae, is well correlated with histopathology. In highly susceptible B. glabrata, for instance, sporocysts and cercariae are observed in great number, simply displacing the structures, in the absence of any host tissue reaction. On the other hand, resistant snails, eliminating few cercariae, exhibit marked focal and diffuse hemocyte proliferation in several organs and tissues, usually resulting in encapsulation of disintegrating sporocysts and cercariae (Lie et al. 1980, Lewis et al. 1993).

We observed that such histological features indeed reflect resistance. However, it is not a fixed characteristic of a given snail strain, but may gradually develop along the course of infection in snails previously presenting a susceptible pattern of cercarial shedding.

A group of highly susceptible B. glabrata from Feira de Santana, BA, Brazil, originated from a single egg, were used. A total of 30 snails were submitted to infection with 15 miracidia, during 50 min with a Feira de Santana strain of S. mansoni (Andrade & Sadigursky 1985). Later, each of them was individually exposed to bright light during 40 min, for cercarial shedding and counting, starting 30 days post-infection and continuing thereafter at every 15th day, up to the 142nd after miracidium exposure. At each experimental point, two infected snails were selected for histological examination, the one representing the highest number of eliminated cercariae and the other, the lowest number in a particular day. These snails were anesthetized with menthol crystals before being taken out of the shell, and immediately fixed in Bouin' s fluid for 4 h. They were then washed in 70% alcohol, dehydrated in absolute alcohol, cleared in xylol and embedded in paraffin. Sections were stained with hematoxylin and eosin. The curve representing cercarial shedding (Fig. 1) started at the 37th day post-infection and reached the highest peak by approximately the 67-82nd day, gradually decreasing toward the 142nd day. Up to the 30th post-infection day no cercarial shedding occurred, but histological examination disclosed numerous multiplying sporocysts at several locations, without tissue reaction. No cercarial differentiation was noted at that time (Fig. 2A). Up to the 67th day, the histological picture of the two snails examined at a time was similar, although the number of cercariae produced could range from 1,047 to 218. In both cases numerous developing sporocysts and cercariae were seen within several organs and tissues, accompanied by minimal focal accumulations of hemocytes around occasionally disintegrating parasites (Fig. 2B). Tissue reactions became more pronounced after the 82nd day, with the formation of several encapsulating lesions, centered by disintegrating sporocysts and cercariae. Toward the end of the experiment, these lesions had became disseminated, especially involving the renal region (Fig. 2C), digestive glands (Fig. 2D) and ovo-testis, while cercarial shedding proportionally decreased.

It is well recognized that, with time, an infected snail may gradually eliminate less and less cercariae. Two possibilities, not mutually exclusive, may explain this situation. The gradual exhaustion of the multiplication capacity of the parasite or the development of a host mechanism of defense, probably due to acquired immunity. The change in host reactivity during the course of infection may, immunopathologically, be interpreted as the development of a strong immunity against the parasite. Susceptibility or resistance of snails to S. mansoni has mainly been claimed to be dependent on host genetic background (Richards 1975a,b) rather than on acquired immunity. Although the capacity to develop acquired immunity may ultimately be related to genetic background, further studies similar to this one, along the lines of infection kinetics in snails susceptible to S. mansoni, may help to better define the role played by, and the factors involved with, acquired immunity in host-parasite relationship.

 

REFERENCES

Andrade ZA, Sadigursky M 1985. Um estudo com-parativo das cepas Feira de Santana (Bahia) e Porto Rico doSchistosoma mansoni na infecção experimetal do camundongo. Mem Inst Oswaldo Cruz 80: 37-40.

Correa MCR, Coelho PMZ, Freitas JR 1979. Susceptibilidade de linhagens de Biomphalaria tenagophila e Biomphalaria glabrata a duas cepas de Schistosoma mansoni (LE-Belo Horizonte, MG e São José dos Campos, SP). Rev Inst Med Trop São Paulo 21: 72-76.

Lewis FA, Richards CS, Knight M, Cooper LA, Clark B 1993. Schistosoma mansoni: analysis of an unusual infection phenotype in the intermediate host snail Biomphalaria glabrata. Exp Parasitol 77: 349-361.

Lie KJ, Jeong KH, Heyneman D 1980. Tissue reaction induced by Schistosoma mansoni in Biomphalaria glabrata. Ann Trop Med Parasitol 78: 157-166.

Newton WL 1952. The comparative tissue reaction of two strains of Australorbis glabratus. J Parasitol 38: 362-366.

Pan CT 1965. Studies on the host-parasite relationship between Schistosoma mansoni and the snail Australorbis glabratus. Am J Trop Med Hyg 14: 931-975.

Richards CS 1975a. Genetic studies of pathologic conditions and susceptibility to infection in Biomphalaria glabrata. Ann New York Acd Sci 266: 394-410.

Richards CS 1975b. Genetic factors in susceptibility of Biomphalaria glabrata for different strains of Schistosoma mansoni. Parasitology 70: 231-241.

Souza CP, Cunha RCP, Andrade ZA 1995. Development of Schistosoma mansoni in Biophalaria tenagophila, Biomphalaria straminea and Biomphalaria glabrata. Rev Inst Med Trop São Paulo 37: 201-206.

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