MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 94(6) Nov/Dez 1999
PAGES: 837-839 DOI: Full paper
Biology of Triatoma pallidipennis Stal 1945 (Hemiptera: Reduviidae:Triatominae) under Laboratory Conditions

José Alejandro Martínez-Ibarra +, Gala Katthain-Duchateau

Laboratorio de Microbiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Apartado Postal 119-4 44100, Guadalajara, Jalisco, México

Abstract

Aspects related to hatching, life time, mortality, feeding behaviour and fecundity for each stage of Triatoma pallidipennislife-cycle were evaluated. The hatching rate observed for 200 eggs was 60% and the average time of hatching was 18 days. Eighty nymphs (N) (40%) completed the cycle and the average time from NI to adult was 168.7±11.7days. The average span in days for each stage was 18.0 for NI, 18.5 for NII, 30.0 for NIII, 35.7 for NIV and 50.1 for NV. The number of bloodmeals at each nymphal stage varied from 1 to 5. The mortality rate was 9.17 for NI, 5.5 for NII, 6.8 for NIII 4.17 for NIV and 13.04 for NV nymphs. The average number of eggs laid per female in a 9-month period was 498.6. The survival rates of adults were 357±217.9 and 262.53±167.7 for males and females respectively.

Triatoma pallidipennis Stal, is one of the most important vectors of Chagas disease in Mexico, occurring in houses and chicken coops in villages of central Mexico (Lent & Wygodzinsky 1979, Zárate & Zárate 1985, Beltrán & Carcavallo 1985, Velasco-Castrejón 1991, Magallón-Gastélum et al. 1998, Martínez-Ibarra et al. 1998, 1999). The biology and behavior of this species have been poorly studied (Maifrino et al. 1989, Martínez-Ibarra et al. 1992, Mena-Segura et al. 1994), in spite of its domiciliarity, frequent contact with man as a blood meal source and high infection rates withTrypanosoma cruzi (Velasco-Castrejón 1991, Magallón-Gastélum et al. 1998, Martínez-Ibarra et al. 1998, 1999, Bautista et al. 1999).

As a part of a larger study on the ecology of T. pallidipennis, studies of the biology of T. pallidipennis under experimental conditions are described.

 

MATERIALS AND METHODS

A laboratory colony established in 1988 from specimens captured in Zacoalco de Torres, Jalisco, was used. The colony was mantained at 25±4ºC and 60±5% relative humidity (rh) and fed every three days on immobilized leghorn hens.

Eggs were grouped by date of oviposition to initiate a cohort of 200 eggs. After eclosion, first-instar nymphs were separated individually into plastic containers (10.5 cm diameter x 20.5 cm heigh), with an upcenter support of absorbent cardboard. Three days after eclosion, the nymphs were individually offered a feed during a 1 hr period until the first blood meal, after that they were offered feed every third day. The bugs were maintained in a dark incubator at 27±2ºC and 60±5% rh, and were checked daily for ecdysis or death.

From the insects that completed development to the adult stage, 15 adult couples were placed in individual containers (10.5 cm diameter x 20.5 cm height) and maintained as previously described to determine adult survival rates and oviposition patterns.

 

RESULTS

Egg eclosion rate was 60% with an average incubation period of 18 days (range 16-27 days). Eighty nymphs completed development to the adult stage (45 males and 25 females) (Table I), taking an average of 2.8 blood meals per nymphal stage (range 1-5) (Table II). The average egg-to-adult development time was 168.7 days (range 131-199) (Table I). The adult survival levels ranged from 27 to 620 days for males (mean 357±217.86) and from 34 to 570 days for females (mean 262.53±167.71). The average number of eggs laid per female was 498.67 (range 408-552).

A partial life table analysis, following the methods of Southwood (1978), indicates a net reproductive rate (Ro) of 145.4 times per generation under these conditions, with an instantaneous daily reproductive rate (r) of 0.062 (Table III).

 

DISCUSSION

Extrapolations from laboratory data to natural populations are relatively easy in domiciliary triatomines, because in nature all life stages occur in a single type of environment and have similar biological requirements. The most important biological factors that are necessary to understand the populations dynamics of any species include: development time and mortality of immature stages; number of female progeny emerged, onset of sexual maturity, fertility and fecundity, mating habits; longevity, and rate of oviposition with respect to feeding frequency (Perlowagora-Szumlewicz 1953). While biological data are available for many species, comparisons among studies are difficult due to wide differences in experimental design, especially with respect to the temperature, humidity (often uncontrolled), feeding frequency, blood meal sources, number of specimens and stage of species studied, density of insects per container, and frequency of observations (Zárate 1983).

The average development time of T. pallidipennis in this study (168.7±11.71days) was shorter than the development time for this species in a previous study (411days) since it was carried out under different feeding frequency (10-12 days) but similar laboratory conditions and source of blood (hens) (Mena-Segura et al. 1994). This is supported by a previous study with other Triatoma species (Galavíz-Silva et al. 1991) where the most frequent feeding the shortest development time. Also, the average development time in our study was similar to other Triatoma species. Zeledón et al. (1970a) observed an average development time of 240 days (range 180-336 days) for T. dimidiata feeding fortnightly on rabbits and maintained at 26.5±0.5oC and 50±5% rh. Rabinovich (1972) found an average development time of 141 days for T. infestans fed weekly on hens and maintained at 26±1oC and 60±10% rh. Zárate (1983) reported an average development time of 143.7 days for females and 205.3 for males of T. barberi fed every 4.2 days on rabbits and maintained at 27oC and 60±10% rh. Ehrenfeld et al. (1998) reported a range of 285-372 of development time for Mepraia (= Triatoma) spinolaifed weekly on chickens and maintained at 28oC and 70% rh.

Egg hatching rates in Triatoma species also vary. They are generally above 80% (Zeledón et al. 1970b, Rabinovich 1972, Zárate 1983, Braga 1998), although lower average hatch rates of 65.9% have been recorded in colonies of T. gerstaeckeri examined under controlled conditions (Galaviz et al. 1991). Egg hatching rate in T. pallidipennis in this study (60%) was similar to that reported by Mena-Segura et al. (1994) in the same species (61.9%), since laboratory conditions were similar.

Mortality showed an irregular pattern. The lowest mortality rate was in the molt from fourth to fifth stage, different to reported data by Mena-Segura et al. (1994) for T. pallidipennis, where mortality was zero in the three youngest nymphal stages; meanwhile the highest was between the fifth to adult, similar to results reported by the same author. In the youngest nymphs, mortality appeared to be due mainly to an incapacity to feed, since dead bugs were generally without significant intestinal content. In contrast, mortality in the older nymphs occurred mainly during their moulting. On average, approximately 75% of stages II-V required two and a half meals in order to molt to the next stage.

The mean adult survival levels (357±17.86) days for males and (262.53±167.71) for females were longer than those reported by Mena-Segura et al. (1994) (182 for males and 225 for females) since feeding frequency was lower than used in our study.

The typical development time and high reproductive rate may reflect how closely the experimental conditions were controlled to match the optimal conditions for this species. The average net reproduction rate (Ro) for T. pallidipenniswas 145.4, considerably higher than those calculated for T. barberi reared under very similar controlled conditions (Ro = 89.1) (Zárate 1983), for T. maculata reared under similar controlled conditions (Ro = 80.5) (Feliciangeli & Rabinovich 1998) and that calculated for T. infestans reared under different controlled conditions (Ro = 25) (Rabinovich 1972).

The development cycle of a triatomine varies according to species, the specific environmental conditions and it is greatly influenced by the availability of blood sources (Schofield 1985).

 

ACKNOWLEDGEMENTS

To Dr Nina Bárcenas and Dr Jesus Romero for help on the revision of the manuscript.

 

REFERENCES

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