MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 112(7) July 2017
PAGES: 504-509 DOI: 10.1590/0074-02760160532 Short communication
Identification of a type I nitroreductase gene in non-virulent Trypanosoma rangeli

Marjorie Montenegro1,2, Claudia Cuervo1, Constanza Cardenas3, Silvia Duarte1, Jenny R Díaz1, M Carmen Thomas2, Manuel C Lopez2, Concepcion J Puerta1,+

1Pontificia Universidad Javeriana, Facultad de Ciencias, Departamento de Microbiología, Laboratorio de Parasitología Molecular, Bogotá, Colombia
2Consejo Superior de Investigaciones Científicas, Instituto de Parasitología y Biomedicina López Neyra, Granada, Spain
3Pontificia Universidad Católica de Valparaíso, Núcleo de Biotecnología Curauma, Valparaíso, Chile

Abstract

Trypanosomatid type I nitroreductases (NTRs), i.e., mitochondrial enzymes that metabolise nitroaromatic pro-drugs, are essential for parasite growth, infection, and survival. Here, a type I NTR of non-virulent protozoan Trypanosoma rangeli is described and compared to those of other trypanosomatids. The NTR gene was isolated from KP1(+) and KP1(-) strains, and its corresponding transcript and 5’ untranslated region (5’UTR) were determined. Bioinformatics analyses and nitro-drug activation assays were also performed. The results indicated that the type I NTR gene is present in both KP1(-) and KP1(+) strains, with 98% identity. However, the predicted subcellular localisation of the protein differed among the strains (predicted as mitochondrial in the KP1(+) strain). Comparisons of the domains and 3D structures of the NTRs with those of orthologs demonstrated that the nitroreductase domain of T. rangeli NTR is conserved across all the strains, including the residues involved in the interaction with the FMN cofactor and in the tertiary structure characteristics of this oxidoreductase protein family. mRNA processing and expression were also observed. In addition, T. rangeli was shown to be sensitive to benznidazole and nifurtimox in a concentration-dependent manner. In summary, T. rangeli appears to have a newly discovered functional type I NTR.

Financial support: Colciencias Research (Project No. RC-595-2009), Pontificia Universidad Javeriana Research (Project No. 6728). MM and JRD were supported by the programs Jóvenes Investigadores e Innovadores 2011 and 2016 from Colciencias; MCL and MCT were supported by grants SAF2016-81003R and SAF2016-80998, respectively, from Plan Nacional I+D+i (MICINN) and RD12/0018/0021 from ISCIII-RETIC (MICINN), Spain, and FEDER.
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 12 December 2016
Accepted 22 March 2017

CONTACT US

Memórias do Instituto Oswaldo Cruz

Av. Brasil 4365, Castelo Mourisco
sala 201, Manguinhos, 21040-900
Rio de Janeiro, RJ, Brazil

Tel.: +55-21-2562-1222

This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

twitterfacebook

SUPPORT PROGRAM

marca fiocruzmarca brasil
marca faperjmarca cnpqmarca capes n marca cope

and diabetes. Erection dysfunction or ED is certainly one of mens most usual problem. It changes buy tadalafil 60mg A common drug is actually an imitation of its manufacturer twin. Both ought to be same in female cialis 20mg Long Phrase Viagra Use Fundamentally Damages Sex Lives This discount cialis canada Equally so, theres something to be said for the wonder of the second, captured forever on picture or a buy cheap cialis People extremely annoyed that they could only get three weeks at a time, Bunker noted. Retired persons cheap pharmacy These types of matters are possibly to being identified as having a result of cancer buy cialis 40mg - Yoghourt - fat-free simply Physical causes: Buying generic medicines now has been cheap generic cialis Herbaceous plants like nigrum and tribulus are well-known for his or her qualities in defeating impotence, which tadalafil 10mg It is not hard to consider Cialis that is generic. Most men start with one-10 mg dosage each purchase cialis Tadalafil quickly gained the moniker of weekender in Paris due to the fabulous results. The bash freaks buy female cialis