MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 112(7) July 2017
PAGES: 520-522 DOI: 10.1590/0074-02760160468 Letter to the editor
Molecular detection of mixed infections with multiple dengue virus serotypes in suspected dengue samples in Tamaulipas, Mexico

Rocío Requena-Castro, Miguel Ángel Reyes-López, Rosa Eminé Rodríguez-Reyna, Prisco Palma-Nicolás, Virgilio Bocanegra-García+

Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, Tamaulipas, México

Abstract

This study aimed to detect dengue virus (DENV) serotypes in serum samples obtained in Matamoros Tamaulipas, Mexico, and to determine the concordance of conventional nested reverse transcriptase polymerase chain reaction (RT-PCR) and a serological test [enzyme-linked immunosorbent assay (ELISA NS1)]. Here, we detected mixed infections consisting of four serotypes of DENV. The most prevalent serotype was DENV-1, followed by DENV-4. This is the first report of DENV-4 in our region. Mixed infections were also detected in 21.5% of samples, and the predominant coinfection consisted of DENV-1 and DENV-2. Therefore, continuous epidemiological surveillance of DENV in this area is required to predict future forms of dengue heterologous infections and the effect of this on health care.

key words:

Financial support: Fondos Mixtos CONACYT (Project Nº 180879).
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 21 October 2016
Accepted 29 March 2017

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