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PAGES: DOI: 10.1590/0074-02760170208 Short communication
First detection of dengue virus in the saliva of immunocompetent murine model

Arthur da Costa Rasinhas1,+, Marcos Alexandre Nunes da Silva1, Gabriela Cardoso Caldas1, Fernanda Cunha Jácome1, Raphael Leonardo1, Flávia Barreto dos Santos2, Priscila Conrado Guerra Nunes2, Ortrud Monika Barth1, Debora Ferreira Barreto-Vieira1

1Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Morfologia e Morfogênese Viral, Rio de Janeiro, RJ, Brasil
2Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Imunologia Viral, Rio de Janeiro, RJ, Brasil


The lack of an experimental animal model for the study of dengue pathogenesis is a limiting factor for the development of vaccines and drugs. In previous studies, our group demonstrated the susceptibility of BALB/c mice to infection by dengue virus (DENV) 1 and 2, and the virus was successfully isolated in several organs. In this study, BALB/c mice were experimentally infected intravenously with DENV-4, and samples of their saliva were collected. Viral RNA extracted from the saliva samples was subjected to qRT-PCR, with a detection limit of 0.002 PFU/mL. The presence of DENV-4 viral RNA was detected in the saliva of two mice, presenting viral titers of 109 RNA/mL. The detection of DENV RNA via saliva sampling is not a common practice in dengue diagnosis, due to the lower detection rates in human patients. However, the results observed in this study seem to indicate that, as in humans, detection rates of DENV RNA in mouse saliva are also low, correlating the infection in both cases. This study reports the first DENV detection in the saliva of BALB/c immunocompetent mice experimentally infected with non-neuroadapted DENV-4.

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Financial support: CNPq, IOC
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Received 30 May 2017
Accepted 4 December 2017