MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 96(7) October 2001
PAGES: 925-926 DOI: Short communication
Dengue Virus Type 3 in Rio de Janeiro, Brazil

Rita Maria R Nogueira +, Marize P Miagostovich, Ana Maria B de Filippis, Maria Aparecida S Pereira*, Hermann G Schatzmayr

Departamento de Virologia, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil
*Secretaria de Saúde de Nova Iguaçu, RJ, Brasil

Abstract

Dengue virus type 3 was isolated for the first time in the country as an indigenous case from a 40 year-old woman presenting signs and symptoms of a classical dengue fever in the municipality of Nova Iguaçu, State of Rio de Janeiro. This serotype has been associated with dengue haemorrhagic epidemics and the information could be used to implement appropriate prevention and control measures. Virological surveillance was essential in order to detected this new serotype.

Dengue fever (DF) and dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) are considered the most medically important and widespread mosquito-borne viral diseases. Dengue (DEN) viruses belong to the familyFlaviviridae, genus Flavivirus, and occur as four antigenically related but distinct serotypes designated DEN-1, 2, 3 and 4 (Westaway et al. 1985). Although all dengue serotypes are responsible for both DF or DHF/DSS, dengue subtyping methods have been used for epidemiologic and pathogenesis investigations, since genetic differences could reveal important information related to the disease (Lanciotti et al. 1994, Rico-Hesse et al. 1997).

In Brazil, since 1986 dengue epidemics have been occurring annually, becoming a national public health problem. A total of 1,832,371 cases were reported, resulting from DEN-1 and/or DEN-2 epidemics, and occurred in 24 out of 26 states and the Federal District/Brasília (Ministry of Health, Brazil). Molecular analysis performed on genome fragments from DEN-1 and DEN-2 viruses identified the Caribbean and Jamaican genotypes, respectively, as the virus strains circulating in the country (Rico-Hesse 1990, Vorndam et al. 1994, Miagostovich et al. 1998). The Jamaica genotype introduction into the Americas resulted in both an increase in the severe forms of the disease and epidemics in which there were a significant number of DHF/DSS cases (Nogueira et al. 1993, Rico-Hesse et al. 1997) .

In January 2001, during a virological surveillance carried out in the municipality of Nova Iguaçu, DEN-3 was isolated from a 40 year-old woman presenting signs and symptoms of a classical DF (WHO 1986). This municipality is located 25 km away from Rio de Janeiro downtown in greater Rio and was the same municipality where DEN-1 was first isolated in 1986 (Schatzmayr et al. 1986). Nova Iguaçu has reported subsequent epidemics of DF, having DEN-1 and DEN-2 viruses isolated during the summer 2000-2001.

DEN-3 virus isolation was performed by inoculation of serum sample into clone C6/36 Aedes albopictus cell line and typed by the indirected fluorescent antibody test using serotype-specific monoclonal antibodies (Gubler et al. 1984). Viral RNAs were extracted from the supernatants of infected cells and from sera using a QIAamp Viral Mini Kit (Qiagen, Inc., Valencia, CA) according to the manufacturer's protocol. The nested RT-PCR (Lanciotti et al. 1992) detected DEN-3 directly from serum sample and confirmed DEN-3 from tissue culture fluid (Figure).

Serological results showed IgM seroconversion by IgM capture enzyme-linked immunosorbent assay (Kuno et al. 1987) while G-ELISA (Miagostovich et al. 1999) characterized dengue primary infection showing titers of <40 and 1/2560 on the acute and convalescent serum, respectively.

The above-mentioned data demonstrate the importance of on-going monitoring of samples of DEN viruses circulating in the country in order to identify the entry of a new serotype or genotype and a potential association between genotype and virulence. The introduction of this new DEN serotype, with a possibility of a large outbreak may represent a new threath, since DEN-3 virus has been associated with the dengue haemorrhagic occurred in India, Sri Lanka and Central America (Lanciotti et al. 1994, Anonymus 1995).

Three more DEN-3 viruses were isolated afterwards in the State of Rio de Janeiro, two of them in the same area of the first isolated strain.

 

ACKNOWLEDGMENTS

To Dr Ari Carvalho de Miranda for encouragement. To Janete Mariotte Lima, Health Department, municipality of Nova Iguaçu. To Eliane Saraiva Machado de Araújo, José da Costa Farias Filho and to José de Carvalho Filho for tecnhical support.

 

REFERENCES

Anonymus 1995. Dengue 3 in Central America. Dengue Branch, San Juan, Puerto Rico. Division of Vector-Borne Infectious Diseases. Dengue surveillance 70: 1-4.

Gubler DJ, Kuno G, Sather E, Valez M, Olivre A 1984. Mosquito cell cultures and specific monoclonal antibodies in surveillance for dengue viruses. Am J Trop Med Hyg 33: 158-165.

Kuno G, Gomez I, Gubler DJ 1987. Detecting artificial anti-dengue IgM immune complexes using an enzyme-linked immunosorbent assay. Am J Trop Med Hyg 36: 153-159.

Lanciotti RS, Calisher CH, Gubler DJ, Chang G-J, Vorndam V 1992. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 30: 545-551.

Lanciotti RS, Lewis JG, Gubler DJ, Trent DW 1994. Molecular evolution and epidemiology of dengue-3 viruses. J Gen Virol 75: 65-75.

Miagostovich MP, Nogueira RMR, Schatzmayr HG, Lanciotti RS 1998. Molecular epidemiology of Den-2 virus in Brazil. Mem Inst Oswaldo Cruz 93: 625-626.

Miagostovich MP, Vorndam V, Araújo ESM, Santos FB, Schatzmayr HG, Nogueira RMR 1999. Evaluation of IgG enzyme-linked immunosorbent assay for dengue diagnosis. J Clin Virol 14: 183-189.

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Rico-Hesse R 1990. Molecular evolution and distribution of dengue viruses type 1 and 2 in nature. Virology 174: 479-493.

Rico-Hesse R, Harrison LM, Salas RA, Tovar D, Nisalak A, Ramos C, Boshell J, De Mesa MTR, Nogueira RMR, Travassos da Rosa A 1997. Origins of dengue type 2 viruses associated with increased pathogenicity in the Americas. Virology 230: 244-251.

Schatzmayr HG, Nogueira RMR, Rosa APAT 1986. An outbreak of dengue virus at Rio de Janeiro. Mem Inst Oswaldo Cruz 81: 245-246.

Vorndam V, Nogueira RMR, Trent DW 1994. Restriction enzyme analysis of American region dengue viruses.Arch Virol 136: 191-196.

Westaway EG, Brinton MA, Gaidamovich SY, Horzinek MC, Igarashi A, Kaariainen L, Lvov DK, Porterfield JE, Russell PK, Trent DW 1985. Flaviviridae. Intervirology 24: 183-192.

WHO 1986. Dengue haemorrhagic fever: diagnosis, treatment and control, World Health Organization, Geneve.

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